Figure 1.

(A) Western blot analysis with antibodies against Hsp70 and Hsc70. Samples were from whole cell extracts of nonstressed control cells (0 h), cells exposed to 30-MPa static (30 sHP) or cyclic (30 cHP) HP for 1–12 h, and cells exposed to heat shock (lane HS) at 43°C for 5 h. Experiments were repeated three times. (B) Analysis of Hsp70 protein accumulation was performed by chemiluminescence reaction, and protein levels were quantitated densitometrically.