Table I.
Web server benchmarking with calcium binding proteins.
| EVALLER | Allermatcha | APPELb | ||||
|---|---|---|---|---|---|---|
| Query protein | Assignmentc (%) | Best hit | Assignmentd (%) | Best hit | Assignment | Best hit |
| Wheat calmodulin | Not an allergen (13) | Bra n 2 | Allergen (45) | Jun o 4 | Non-allergen | NA |
| Rice calmodulin | Not an allergen (13) | Bra n 2 | Allergen (43) | Jun o 4 | Non-allergen | NA |
| Rape calmodulin | Not an allergen (13) | Bra n 2 | Allergen (46) | Jun o 4 | Non-allergen | NA |
| Mouse calmodulin | Not an allergen (13) | Bra n 2 | Allergen (43) | Jun o 4 | Non-allergen | NA |
| Human CaBP5 | Not an allergen (9) | Ani s Troponin | Allergen (36) | Cyn d 7 | Non-allergen | NA |
| Human calmodulin-like | Not an allergen (13) | Bra n 2 | Allergen (45) | Che a 3 | Non-allergen | NA |
| Human calmodulin | Not an allergen (13) | Bra n 2 | Allergen (43) | Jun o 4 | Non-allergen | NA |
| Bovin CaBP5 | Not an allergen (9) | Ani s Troponin | Allergen (35) | Cyn d 7 | Non-allergen | NA |
| Banana calmodulin-like | Not an allergen (13) | Bra n 2 | Allergen (46) | Jun o 4 | Non-allergen | NA |
| Arabidopsis calmodulin-like | Not an allergen (13) | Bet v 4 | Allergen (44) | Che a 3 | Non-allergen | NA |
| Apple calmodulin | Not an allergen (13) | Bra n 2 | Allergen (45) | Jun o 4 | Non-allergen | NA |
| Tobacco polcalcin 1 | Allergen (0.3) | Ole e 3 | Allergen (100) | Self | Allergen | NA |
| Tobacco polcalcin 2 | Aallergen (0.3) | Ole e 3 | Allergen (100) | Self | Allergen | NA |
| Lilac polcalcin (Syr v 3) | Allergen (0) | Bet v 4 | Allergen (100) | Self | Allergen | NA |
cThe percentage indicates an uncertainty score for the assignment. (See ‘Material and Methods’ section for details.)
dThe percentage indicates the sequence identity of the best 80-mer match, as revealed by alignment (decision threshold is 35%). This is one of the two criteria for bioinformatics assessment of potential allergenicity, as appearing in the Codex Alimentarius guidelines, the other one being scanning for presence of short identical peptide matches.