Fig. 2.
Phylogenetic tree of YJM-GNAT homologs. (A) YJM-GNAT homologs were retrieved by BLASTP against the nonredundant database by using the threshold (E value of ≤1 × 10−5, identity of ≥30%, and the alignment matching at least 75% of the length of both query and subject sequences). Representative species are shown. Multiple alignments were built by CLUSTALW. A phylogenetic tree was then constructed by using the neighbor-joining method of the PHYLIP package. GNAT homologs are represented by their species names. (B and C) Phylogenies of two other YJM789 genes encoding acetyltrasferases for comparison: ELP3 (B) and ECM40 (C).