FIG. 2.

Inhibitory activities of compounds 1 to 4 for the NS5B mutant panel. (A to D) In vitro activity of the NS5BΔ21 P495L, M423T, M414T, or S282T mutant with compounds 1 to 4. IC₅₀ n-fold changes are calculated with respect to the obtained IC₅₀ of NS5BΔ21 1b J4 for each class of compound. Light-gray bars, benzimidazole 1; Cross-hatched bars, thiophene 2; white bars, benzothiadiazine 3; black bars, N-acyl pyrrolidine 4. Compounds with an IC₅₀ n-fold change greater than 100 were considered to be inactive (dotted line).