TABLE 1.

Primers used in this study

Identifier	Sequencea
Subtype-specific primers used in reverse transcription
HCV1a-RT	5′-AGG CCG GAG TGT TTA CCC CAA C-3′
HCV1b-RT	5′-GGC CTG GAG TGG TTA GCT CCC C-3′
HCV2a-RT	5′-GTT AGC TAT GGA GTG TAG CTA-3′
HCV2b-RT	5′-ATG GAG TGT AGC TAG GGT TTG CC-3′
HCV3a-RT	5′-GAA ATG GAG TGT TAT CCT ACC-3′
HCV4a-RT	5′-CCT AAG GTC GGA GTG TTA AG-3′
HCV5a-RT	5′-GGA GTG TTT AGC TCC CAG C-3′
HCV6a-RT	5′-GAG TGT TGC TAA GGC CGC TCG-3′
Primers used for NS5B cloning
FP1b_J4	5′-TAG GGC GCT AGC TCA ATG TCC TAT ACG TGG ACA GGC GCC-3′
RP1b_J4	5′-TAG GGC CTC GAG GCG GGG TCG GGC ACG AGA CAG GCT GTG-3′
FP1a	5′-TAG GGC GCT AGC TCA ATG TCT TAT TCC TGG-3′
RP1a	5′-TAG GGC CTC GAG GCG GGG CCG GGC ATG-3′
FP1b	5′-TAG GGC GCT AGC TCA ATG TCC TAC ACA TGG-3′
RP1b	5′-TAG GGC CTC GAG GCG GGG TCG GGC ACG-3′
FP2a	5′-TAG GGC GCT AGC TCC ATG TCA TAC TCC TGG-3′
RP2b	5′-TAG GGC CTC GAG GCG GGG TCG GGC ATG-3′
FP3a	5′-TAG GGC GCT AGC TCT ATG TCG TAC TCT TGG-3′
RP3a	5′-TAG GGC CTC GAG GCG GGT TCG GGC ACG-3′
FP4a	5′-TAG GGC GCT AGC TCG ATG TCA TAC TCG TGG AC-3′
RP4a	5′-TAG GGC CTC GAG GCG GGG TCG GGC ATG-3′
FP5a	5′-TAG GGC CAT ATG TCC ATG TCA TAC ACC TGG ACT-3′
RP5a	5′-TAG GGC GCG GCC GCG CGG GGT CGG GCA CG-3′
FP6a	5′-TAG GGC CAT ATG TCC ATG TCC TAT TCC TGG ACG-3′
RP6a	5′-TAG GGC GCG GCC GCA CGG GGT CGG GCC TG-3′

^aNheI, XhoI, NdeI, and NotI sites of primers used for NS5B cloning are shown in bold.