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. 2007 May 2;81(13):7178–7188. doi: 10.1128/JVI.00404-07

FIG. 5.

FIG. 5.

The mitochondrial localization of ORFV125 is necessary for its antiapoptotic function. 143B cells were mock transfected (PBS) or transfected with the indicated GFP fusion constructs and UV irradiated either 30 h (A and C) or 12 h (B) later. After a further 17 h (A and C) or 12 h (B), the DNA content of permeabilized, PI-stained cells was examined by flow cytometry. The shortened time between transfection and harvesting in panel B was used to avoid degradation of the ORFV125Δts construct within the cell. The percentage of hypodiploid (apoptotic) cells in each population was calculated. The data presented are averages of three independent experiments with standard deviations indicated. Asterisks indicate UV-irradiated samples in which the apoptotic population is significantly reduced (A, P < 0.05 for ORFV125wt and P < 0.01 for Bcl-w and Bcl-2; B, P < 0.05; C, P < 0.001 for ORFV125wt and P < 0.01 for ORFV125AAA) compared with the corresponding UV-irradiated mock-transfected (A and B) or ORFV125ts-transfected (C) sample.