FIG. 6.

ORFV125 inhibits cytochrome c release, but not JNK phosphorylation, following UV treatment. Stable 143B cell lines expressing ORFV125, Bcl-2, or the empty vector were either mock treated (−UV) or UV irradiated. (A) Cells were fractionated into cytosolic and mitochondrial fractions after 4, 6, or 8 h. Release of cytochrome c from the mitochondria into the cytosol was determined by Western blot analysis with an anti-cytochrome c antibody. This blot is representative of three independent experiments. (B) Cell lysates were prepared after the indicated times. Cells harvested at 0 min were collected immediately after UV exposure. Immunoblot analysis was performed to compare the levels of two isozymes of phosphorylated JNK (p46-JNK1 and p54-JNK2) with total JNK. The latter also served as a loading control.