FIG. 1.

KSHV infection increases HUVEC invasion of the extracellular matrix. (A and B) Mock- or KSHV-infected HUVEC were assayed for their ability to invade a Matrigel layer at 24 hpi. A representative illustration (A) and quantification (B) of mock- or KSHV-infected HUVEC that had invaded the Matrigel layer are shown. (C) HUVEC invasiveness assayed at different time post-KSHV infection. All the invasion assays were carried out for 24 h as described in Materials and Methods. Mock- or KSHV-infected HUVEC that had invaded the Matrigel layers were quantified in three independent experiments, each with three to five repeats (A and B). The total number of repeats (n) for each condition is shown. Each dot represents the average number of cells per microscopic field in one test well in the invasion assay. The bars and their associated numbers are the average numbers of invaded cells per field from all the wells under different test conditions. P values shown are from a two-tailed Fisher's exact test.