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Feline cells expressing HIV receptors do not support spreading replication of HIV-1. (A) HOS.CD4.CCR5, KE-R.CD4.CCR5, and CrFK.CD4.CCR5 cells were infected with R5-tropic HIV-1NL-Bal at an MOI of 0.05. Supernatant virions were quantified by p24 ELISA at the indicated days. (B) HIV-1NL-Bal-infected feline cells, KE-R.CD4.CCR5 cells, and CrFK.CD4.CCR5 cells showed syncytium formation (arrows) at 3 to 4 dpi (magnification, ×10).
