TABLE 1.
Sequence characteristics of HIV-Luc DNA genomes of virions derived from CrFK cells and 293T-expressing feline APOBEC3 proteins (feA3C, feA3H, feA3CH) or empty expression plasmid
| Producer cell and protein or plasmid (vector) | No. of sequences analyzed | No. of G→A mutations/no. of other mutations | No. of mutations per 100 nucleotides | No. of clones without G→A editing | Minimal no. of G→A per clone | Maximal no. of G→A per clone | Average no. of G→A per clone | No. of G→A exchanges per 100 Gs | No. of G→A exchanges per 100 nucleotides |
|---|---|---|---|---|---|---|---|---|---|
| CrFK | |||||||||
| Vector | 10 | 47/14 | 0.94 | 0 | 4 | 7 | 4.7 | 2.7 | 0.72 |
| 293T | |||||||||
| Vector | 10 | 0/7 | 0.11 | 10 | 0 | 0 | 0 | 0 | 0 |
| feA3C | 10 | 2/3 | 0.08 | 8 | 1 | 1 | 0.2 | 0.1 | 0.03 |
| feA3H | 10 | 70/9 | 1.22 | 1 | 4 | 16 | 7 | 4.0 | 1.08 |
| feA3CH | 10 | 99/5 | 1.60 | 0 | 4 | 17 | 9.9 | 5.6 | 1.52 |