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Northern blot analysis of icTS-LA6. Cultures of cells were infected at an MOI of 1 PFU/cell, and intracellular RNA was isolated at 8 h p.i. The RNA was separated on 1% agarose gels, blotted onto a nitrocellulose membrane, and probed with an MHV N-gene-specific probe. icTS-LA6 and wt-icMHV grown at the permissive temperature of 32°C generate equivalent quantities of subgenomic RNA at 8 h p.i. Numbered bands correspond to mRNAs 1 to 7.
