FIG. 6.

Processing of nsp10 and the structural proteins. Immunoprecipitations were conducted to evaluate the processing of nsp10 and the structural proteins S, M, and N. Cells were infected at an MOI of 2 PFU/cell with infections initiated and maintained at the permissive temperature. (A) Anti-nsp10 antibody was used to immunoprecipitate proteins from the cytoplasmic fraction of cells. nsp10 was detected and processed at the permissive temperature, as was the p150 intermediate. (B) Anti-MHV antibody was used to detect structural proteins in wt-icMHV grown at 37°C and compared to icTS-LA6 and TS-LA6 grown at 32°C.