TABLE 1.

Primers used for engineering of nsp10 mutants and RT-PCR

Primer	Sequence (5′-3′)	Sense	Purpose
icTS-LA6S	GAAGACGAAGATTCTTATGGTGGTGCTTCC	+	Mutant icTS-LA6
icTS-LA6A	GAAGACGAATCTTCATTAGTGGTTGCCTCCGGC	−	Mutant icTS-LA6
nsp10-WS	ACAGGGTGGAGTTCCCGTTA	+	PCR and sequencing of nsp10
nsp10-WA	CCTAAGGGCACTTGGACAAA	−	PCR and sequencing of nsp10
Sg-N1S	AAGAGTGATTGGCGTCCGTA	+	RT and quantitative PCR
Sg-N1A	AGCGCGGTTTACAGAGGAG	−	RT and quantitative PCR