Figure 1.

Growth response of PR1 cells to E2 and antiestrogen ICI. (A) Confluent PR1 cells were preincubated in DMEM-DCC-FBS medium for 5 days. Cells (250,000) were incubated either with 1 nM E2 or 1 nM ICI in 6-well plates. For the control group, vehicle solution (ethanol) was added. The medium was changed every day and cell numbers were counted by hemocytometer. (B) For DNA quantification, cells were lysed and genomic DNA was isolated by a Purgene genomic DNA isolation kit. Each point is the mean ± SD of triplicate samples.