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. 2007 Apr 18;81(13):7300–7305. doi: 10.1128/JVI.02406-06

FIG. 1.

FIG. 1.

Generation of MHV-68 oriLyt mutants. (A) Scheme of the mutants generated. “P” indicates the probes used for Southern blot analysis, corresponding to nucleotides 25,097 to 25,833 (P1), 101,141 to 102,045 (P2), and 101,443 to 102,699 (P3). (B) Restriction enzyme and Southern blot analyses of BAC DNAs digested with the restriction enzyme SacI and hybridized with probes P1 and P2. (C) Restriction enzyme and Southern blot analyses of BAC DNAs digested with the restriction enzyme KpnI and hybridized with probe P1. (D) Restriction enzyme and Southern blot analyses of BAC DNAs digested with the restriction enzyme BglII and hybridized with probe P3. Lanes P and A to E, parental and mutant BACs, as indicated in panel A and Table 1. The expected fragments are indicated by arrows on the right. Marker (M) sizes (in kilobase pairs) are indicated on the left.