FIG. 4.

Sorting and infectivity profiles of CD1a^hi and CD1a^lo/neg DC-100 cells. (A) DC-100 cells were stained with FITC-conjugated CD1a antibody and sorted to isolate CD1a^lo/neg and CD1a^hi DC-100 cells. From left to right, dot plots show unsorted, CD1a^hi, and CD1a^lo/neg DC-100 cells. (B and C) Luciferase activity measured in cocultures of unactivated (closed squares) and activated (open squares) CD1a^hi LCs with target cells. Background levels were defined by virus-exposed p4-2 targets (asterisks). (B) Cocultures of unactivated (closed diamonds) and activated (open diamonds) CD1a^lo/neg cells; (C) cocultures of immature (closed triangles) and mature (open triangles) MDDCs. Each data point represents the average of three samples with error bars representing the standard errors of the means. Each graph is representative of three independent experiments.