FIG. 5.
Enhanced transinfection by activated LCs involves internalized virions. (A) Luciferase activity measured in cultures of p4-2 target cells with unactivated and activated CD1ahi LCs that were previously exposed to virus and washed to remove unbound virus. Immature and mature MDDCs were also pulsed with virus and served as negative and positive controls, respectively. (B) Unactivated and activated LCs were pulsed with virus for 2 h, washed to remove unbound virus, and then exposed to 0.05% trypsin plus 0.53 mM EDTA for 5 min at 37°C to remove surface-bound virions. Luciferase activity was measured as described above. Each data point represents the average of three samples with error bars representing the standard errors of the means. Representative results from four independent experiments are shown.