FIG. 6.

Characterization of MVB components in activated LCs. Unsorted unactivated and activated DC-100 cells were incubated with X4-tropic HIV with RFP-Vpr prior to fixing and staining. (A) Percentage of unactivated (light bars) and activated (dark bars) LCs with the representative tetraspanin associated with HIV. Error bars show standard errors of the means. (B) LCs were identified by positive staining for CD1a (blue). Virus was identified by expression of fluorescently tagged Vpr (pseudocolored green), and cells were then stained for CD81 (red). The boxed area is enlarged and color separated to show individual stains.