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. 2007 Apr 25;81(13):7077–7085. doi: 10.1128/JVI.00058-07

FIG. 3.

FIG. 3.

Binding of UL84 to SL-RNA is dependent on oligonucleotide secondary structure. (A) Autoradiograph of dried agarose gel showing heat denaturation of SL-RNA. Lanes: 1, no protein; 2, 3 μl UL84 plus nondenatured SL-RNA; 3, 3 μl UL84 plus nondenatured SL-RNA and UL84 MAb; 4, 1 μl UL84 plus denatured SL-RNA; 5, 5 μl UL84 plus denatured SL-RNA; 6, 10 μl UL84 plus denatured SL-RNA; 7, 1 μl UL84 plus denatured SL-RNA and UL84 MAb; 8, 5 μl UL84 plus denatured SL-RNA and UL84 MAb; 9, 10 μl UL84 plus denatured SL-RNA and UL84 MAb. (B) Autoradiograph of dried polyacrylamide gel showing the same samples deproteinized. SS, supershifted band upon incubation with anti-UL84 antibody; S, shifted UL84-SL-RNA species.