TABLE 4.

Determining the PCR subcloning fidelity of the seven-G homopolymer region in WT HSV-1 tk

PCR template	No. of clones sampled	No. of clonesa with:					% of mutant clones
		7-G (WT) sequence	1-G del	1-G ins	2-G ins	3-G ins
LT6 DRG DNA from patient 708	46	38	0	2	5	1	17
Plasmid with WT tk	43	43	0	0	0	0	0.0b
TG DNA from patient 725	24	24	0	0	0	0	0.0c

^adel, deletion; ins, insertion.

^bThe difference between the results for the WT tk control template and the template from patient 708 were statistically significant (P = 0.006).

^cThe difference between the results for the template from patient 725 and that from patient 708 were statistically significant (P = 0.04).