FIG. 2.

Both the proximal and distal parts of the CT region contribute to dimerization. (A) Schematic diagram showing sequence of the truncation mutants spanning the zipper and CT regions. The zipper region is shown as an open box and the CT region as a cross-hatched box. (B) Amounts of translation product generated from wheat germ extract for Zta and Zta truncated mutants used in the dimerization assay were assessed by SDS-PAGE and quantitated by phosphorimaging. (C) Equivalent amounts of each protein were incubated at 37°C and cross-linked by addition of 0.05% glutaraldehyde for 30 min. The reaction was quenched by the addition of glycine, and dimeric proteins were separated on a 10% bis-Tris gel and visualized using phosphorimaging. A representative gel is shown.