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Requirement of the proximal CT region for interaction with three ZREs. (A) Proteins were assessed by SOS-PAGE. Abilities of indicated Zta truncation mutants to bind to ZREs were determined by EMSA analysis. In all cases the probe was in excess. Following separation of free and bound ZREs by electrophoresis, locations of the complexes were determined using phosphorimaging, with subsequent quantitation. Duplicate experiments were undertaken, and representative images are shown (B).
