FIG. 7.

CVB3 2B and 2BC expression focuses endocytic vesicles to the Golgi compartment. (A) Cells were transfected with each EGFP(mem) dicistronic construct, and at 16 h posttransfection, endosome localization was tracked using the lipophilic styryl dye AM4-65, which fluoresces red (first column) and allows the localization of newly endocytosed vesicles. Nuclei (DAPI staining) and EGFP(mem) signals are depicted in columns two and three, respectively. (B) The ImageJ “analyze particles” feature was utilized to determine the total area of AM4-65 fluorescence in relationship to the total area of the cell across a minimum of 50 cells from three independent experiments. These values were graphed and their standard deviations calculated (error bars). Student's t test was done to compare each of the three transfections to the values for the control cell population, and P values are given above the bars.