TABLE 1.
Oligonucleotides used for the site-directed mutagenesis
| Amino acid changea | Nucleotide sequence (5′-3′)b |
|---|---|
| SaV polyprotein | |
| H1069A | CACATCTGGGGGTGATgccATTGGGTATGGTTG |
| H1075A | CATTGGGTATGGTTGTgccATGGGTAATGGGGTG |
| H1086A | GTTGTCACAGTTACAgccGTGGCCTCTGCGTCTG |
| E1107A | CAGGAAGACCGAGGGTgcaACCACCTGGGTGAAC |
| H1120A | CTTGGTCACTTGCCCgccTACCAGATCGGTGATG |
| H1136A | CTACTACTCGGCGCGCCTAgccCCTGTCACCACGC |
| E1143A | GTCACCACGCTTGCGgcgGGGACGTATGAG |
| E1147A | GCGGAGGGGACGTATgcgACACCCAATATCACG |
| H1186A | CGTTTGGTCGGACTGgccGCAGCCACATCAAC |
| FCV polyprotein | |
| C1193A | ACTCACCCTGGAGATgccGGGTTGCCGTATATTGATGAT |
| H1079A | GGGCCTGGCACTAAATTTgccAAAAATGCCATTGGATCAG |
| H1093A | GATGTGTGTGGGGAGgccAAGGGTTACTGCGTCCAC |
| H1099A | CACAAGGGTTACTGCGTCgccATGGGTCATGGGGTCTATG |
| H1102A | GGTTACTGCGTCCACATGGGTgctGGGGTCTATGCCTCTG |
| H1110A | TATGCCTCTGTGGCAgccGTGGTGAAAGGTGATTCCTAT |
| E1121A | GATTCCTATTTCTTGGGTgccAGGATCTTTGATGTAAAAACC |
| D1125A | GGTGAAAGGATCTTTgccGTAAAAACCAATGGTGAATTCTG |
| E1131A | GTAAAAACCAATGGTgccTTCTGTTGTTTCCGAAGCACT |
| D1155A | GGGAAACCAACCCGCgccCCATGGGGGTCGCCAGTTG |
| E1164A | TGGGGGTCGCCAGTTGCAACAgcgTGGAAACCAAAGGCCTATAC |
| H1208A | AGGGTTACCGGCTTAgccACTGGATCTGGTGGACCCAAA |
a
Amino acids are shown in the one-letter code. Letters before the numbers indicate the original amino acid residues, and letters after the numbers indicate the mutant amino acid residues.
b
Only the positive-sense oligonucleotide sequences are shown. The codons corresponding to the changed amino acids are indicated as lowercase letters.