FIG. 4.
Binding of SeV NTAIL to PX is not influenced by ionic strength. Shown are the results of Coomassie blue staining by 15% SDS-PAGE. Lane M, size markers indicated in kilodaltons. The two following lanes contain purified PX and NTAIL proteins as specific markers. The next two lanes show two control experiments in which immobilized tagged PX on nickel beads was incubated without NTAIL and NTAIL was incubated without PX. Coelution experiments were performed with immobilized tagged PX incubated with NTAIL and washed with different NaCl concentrations (0, 100, 300, and 500 mM).