FIG. 8.

Characterization of N_TAIL(443-501) free and in complex with PX. (A) Secondary C^α chemical shifts, where high positive values indicate helical propensity, (B) hetNOE, (C) λ_NOE, and (D) H^N-H^N NOEs of N_TAIL(443-501) free. For the i,i + 1 NOEs in panel D, the thickness of the line indicates strong, medium, or weak. (E) Backbone and arginine side-chain combined NH chemical shift changes upon interaction with PX. Chemical shift changes superior to 0.045 ppm (dashed line) were considered significant. Open bars in panels B, E, and F are from arginine side-chain N^ɛH^ɛ resonances. (F and G) hetNOE (F) and HET-SOFAST (G) of N_TAIL(443-501) in complex with PX. (H) H^N-H^N NOEs in complex with PX. Note that several data points are missing, as several resonances disappeared upon interaction with PX. The gray area highlights the region affected by PX binding.