TABLE 1.
Characteristics of GP1 mutant pseudotyped FIV particles
| Mutation(s) | GP1 incorporationa | Transduction efficiency (%)b |
|---|---|---|
| N40A/S41A | +++ | 171.8 ± 43.9 |
| S58A/S59A | +++ | 80.9 ± 9.7 |
| G67A/L68A | +++ | 192.6 ± 30.3 |
| G74A/V75A | +++ | 359.9 ± 51.3 |
| T77A | +++ | 129.7 ± 12.3 |
| A82S | +++ | 134.3 ± 9.0 |
| T83A | +++ | 183.7 ± 20.4 |
| K84A | ++ | 55.4 ± 4.9 |
| R85A | ++ | 147.6 ± 24.2 |
| W86A | +/− | 0 ± 0 |
| G87A/F88A | +++ | 0 ± 0 |
| S90A | +++ | 142.1 ± 2.5 |
| P94A | +++ | 97.1 ± 14.5 |
| V96A/V97A | +++ | 383.3 ± 16.9 |
| V97A | +++ | 160.6 ± 20.6 |
| N98A | +++ | 96.9 ± 6.5 |
| E106A/N107A | +++ | 181.5 ± 16.3 |
| Y109A/I113T | +/− | 0.4 ± 0.4 |
| K114A/K115A | +++ | 2.1 ± 0.6 |
| S119A | +++ | 63.6 ± 12.8 |
| P126A/D127A | +++ | 100.6 ± 41 |
| P133A | +++ | 280.0 ± 32.4 |
| R134A | +++ | 65 ± 24 |
| R136A/Y137A | +++ | 144.6 ± 46.3 |
| K140A | +++ | 25.1 ± 12.8 |
| G143A | +++ | 1 ± 0.2 |
| T144A | +++ | 182.4 ± 46.7 |
| P146A/C147A | +++ | 0.2 ± 0.2 |
| D150A | +++ | 67.9 ± 12.4 |
| F153A/H154A | +++ | 0 ± 0 |
| K155A | +++ | 109.4 ± 12.5 |
| E156A | +++ | 94.0 ± 9.7 |
| G157A | +++ | 157.8 ± 10.7 |
| A158S | +++ | 141.0 ± 12.9 |
| F159A | ++ | 0 ± 0 |
| F160A | + | 26.6 ± 13 |
| Y162A | ++ | 44.3 ± 12.7 |
| D163A | +++ | 98.2 ± 14.0 |
| R164A/L165A | ++ | 52.2 ± 24.4 |
| A166S | +++ | 163.9 ± 12.1 |
| S167F/R172A | +++ | 171.1 ± 26.5 |
| T168A | +++ | 218.4 ± 7.1 |
| V169A | +++ | 149.3 ± 12.3 |
| G173A | +++ | 199.3 ± 13.3 |
| T174A | +++ | 134.7 ± 24.6 |
| T175A/F176A | +/− | 0.5 ± 0.3 |
| A177S | +++ | 99.1 ± 6.9 |
| E178A | +++ | 139.7 ± 22.1 |
| G179A | +/− | 7.5 ± 4.4 |
| L186A/P187A | +++ | 138.6 ± 14.5 |
| S196A/S197A | ++ | 104.7 ± 26.8 |
| S212A/G213A | +++ | 159.2 ± 71.4 |
| Y214A | +/− | 0.1 ± 0.1 |
| Y221A/Q222A | +/− | 1.7 ± 1.1 |
| E230A/T231A | +++ | 170.8 ± 38.9 |
| T240A/Y241A | +++ | 232.1 ± 33.8 |
| P251A/Q252A | +/− | 1.3 ± 0.6 |
| T260A/I261A | +/− | 32.6 ± 12.9 |
| N269A/T270A | +/− | 24.7 ± 7.8 |
| G287A/E288A | +++ | 147.8 ± 13.3 |
| W292A/E293A | +++ | 115.9 ± 19 |
| K296A/N297A | +++ | 155.4 ± 20.8 |
| E305A/E306A | ++ | 37.8 ± 0.7 |
a
Mutant GP1s were examined for incorporation into virion particles by immunoblotting and were compared to the wild type. +++, ≥80% of wild type; ++, ≥50% of wild type; +, ≥25% of wild type; +/−, <25% of wild type.
b
Transduction efficiency was determined on permissive SNB-19 cells with virion input normalized for RT activity.