FIG. 3.
In vitro cytotoxicity of CTLs against the AAV2 capsid. Mice were immunized with DCs infected with Ad/cap2 or pulsed with rAAV2/GFP or just control medium (Materials and Methods). Ten days after the last vaccination, spleen cells (n = 3) were separated and restimulated with DCs infected with the groups mentioned above for 5 days in the presence of IL-2 at 10 ng/ml. Spleen cells before or after restimulation were used as effectors. CT26/luc cells transduced by rAAV2/gluc at 106 particles/cell were used as targets. The effector/target ratio is depicted as well as cytotoxicity, which is inferred by decreased luc activity. (A) Cytotoxicity of unstimulated spleen cells with an extended coculture period (48 h). (B) Cytotoxicity of restimulated spleen cells after a 4-h incubation with target cells. A representative experiment of three independent experiments is shown.