FIG. 2.

Enhanced viral transmission of mMDDCs depends on the amount of HIV_{JRFL/NL43-Luc} actively captured. (A) Comparative capture at 37°C and binding at 4°C of HIV_{JRFL/NL43-Luc} by distinct cell types. A total of 3 × 10⁵ MDDCs, Raji DC-SIGN cells, and Raji cells were incubated at 37°C (dark bars) or 4°C (clear bars) with 300 ng of HIV_{JRFL/NL43-Luc} p24^gag in a final volume of 0.3 ml, washed five times with PBS, and lysed with 0.5% Triton (at a final concentration of 5 × 10⁵ cells per ml) to measure p24^gag by ELISA. (B) HIV_{JRFL/NL43-Luc} transmission to Hut CCR5⁺ target cells of pseudotyped virus captured at 37°C (dark bars) or bound at 4°C (clear bars). Different cell types pulsed as described above (A) were cocultured with target cells at 37°C and assayed for luciferase activity 48 h postpulse. A and B show a representative experiment including mean values and SEM for three different donors.