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. 2007 May 9;81(14):7584–7597. doi: 10.1128/JVI.02616-06

FIG. 2.

FIG. 2.

TRAIL and TRAIL death receptor (DR4 and DR5) expression in HIV- or SIV-exposed PBMC. (a) Percentage of mTRAIL+ PBMC in PBMC from human (closed symbols) and chimpanzee (open symbols) PBMC during 10 days of culture. The data represent the means ± standard errors (SE) of five experiments. (b) Percentage of human CD4+ T cells, monocytes (CD14+), and iDC (CD11c/HLA-DR+) expressing mTRAIL after HIV infection within each population in the total PBMC cultures. The data represent the means ± SE of three experiments. (c) Analysis of TRAIL receptors DR4 and DR5 in human and chimpanzee PBMC infected with HIV. Mean percentages ± SE of cells expressing the receptor are reported for total PBMC and for CD4+ T cells present in the PBMC culture (data derived from three independent donors). (d) Effect of antibodies against TRAIL and TRAIL receptors on HIV-induced PBMC apoptosis, showing the percentage of apoptotic human CD4+ T cells present on day 7 of culture under different conditions. Five experiments were carried out, and the average percentages of apoptosis observed for the mock-treated samples were subtracted from the other experimental values. (e) Effect of antibodies against CD95, CD95L, IFN-α, and IFN-αR on HIV-induced CD4+ T-cell apoptosis (mean percentages of three independent samples). Rates of HIV or SIV infection shown by ICS on day 7 in all the virus-infected cultures were comparable.