TABLE 2.
Susceptibility of cell lines to infection with PRRSV genotypes I and IIa
| Cell line | Susceptibility to PRRSV isolate
|
|||||
|---|---|---|---|---|---|---|
| Genotype I, EU98V226 | Genotype II
|
|||||
| P129 | P201 | P1151 | NVSL 94-3 | IND5 | ||
| FK-D4 | ++ | + | +++ | +++ | ++ | ++++ |
| PK-9 | + | + | ++ | + | + | ++ |
| PK-A10 | + | + | ++ | ++ | ++ | ++ |
| MARC-145 | ++ | + | +++ | + | ++++ | +++ |
| Parental PK032495 | − | − | − | − | − | − |
| Parental NLFK | − | − | − | − | − | − |
a
Cells were infected with various PRRSV isolates. At 3 days postinfection, cells were fixed and stained with MAb SDOW-17-FITC and examined under a fluorescence microscope for nucleocapsid expression. ++++, many positive cells; +++, moderate number of positive cells; ++, some positive cells; +, very few positive cells; −, negative.