FIG. 7.

Effects of hantavirus infection on the liver. An alkaline phosphatase immunohistochemical assay using anti-nucleocapsid antibody was performed on liver samples from ANDV-infected (animal 207) (A) or SNV-infected (animal 259) (B) animals at 14 days postinfection. For panels A and B, the bars represent 30 μm. (C) Ultrastructural analysis revealed large nucleocapsid inclusions within hepatocytes of the ANDV-infected hamsters. (D) Immunoelectron microscopy using anti-SNV nucleocapsid antibody confirmed that the inclusion bodies contained hantavirus antigen. Original magnification, ×80,000 (animal 227). (E) Serum chemistries from ANDV- and SNV-infected hamsters were determined using a General Chemistry 12-disc panel rotor and a Piccolo point-of-care blood analyzer. Error bars represent the SEM. All bilirubin values were identical and within normal limits, as expected in the absence of significant liver damage. Normal reference ranges are shown in brackets. In, inclusion; *, no data; ^★, below limit of detection.