FIG. 5.

Anti-HSV activity of Spm8CHAS if present throughout the infection. CaSki cells were pretreated with the indicated dose of Spm8CHAS or PBS for 30 min and then challenged with serial 10-fold dilutions of HSV-2(G). After 1 h of incubation, the inoculum was removed and cells were overlaid with serum-free medium in the presence or absence of the drug at the indicated concentrations. Plaques were counted 48 h postinfection; only wells containing 25 to 100 plaques were used to calculate the viral titer. The results are the mean viral titer (PFU/ml) ± the standard deviation obtained from two independent experiments conducted in duplicate. The asterisks denote a significant reduction in the viral titer compared to the titer obtained in the presence of control buffer (P < 0.01).