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Early activation of JNK and subsequent c-Jun phosphorylation in PV-infected neuronal cells. Activation of each JNK and c-Jun was analyzed in whole-cell lysates at the indicated times p.i. by Western blotting using specific anti-phospho (Thr183/Tyr185)-JNK (p46 [JNK1] and p54 [JNK2/3]) and anti-phospho (Ser63)-c-Jun antibodies. Blots were subsequently stripped and reprobed with antibodies recognizing all JNK and c-Jun forms to confirm equal protein loading.
