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. 2007 May 9;81(14):7504–7516. doi: 10.1128/JVI.02690-06

FIG. 7.

FIG. 7.

UV-inactivated PV induces early JNK activation but not cell death in IMR5 cells. (A) UV-inactivated PV induces early JNK activation in IMR5 cells. JNK activation was analyzed by Western blotting whole-cell lysates from cells infected with infectious or UV-inactivated PV (30 min p.i.) with specific anti-phospho (Thr183/Tyr185)-JNK antibody. Blots were subsequently stripped and reprobed with antibodies recognizing all forms of JNK to confirm equal protein loading. (B) UV-inactivated PV did not induce cell death in IMR5 cells. Mock-infected IMR5 cells and cells infected with infectious PV or UV-inactivated PV (8 h p.i.) were analyzed by flow cytometry after AO staining and the increase (n-fold) in apoptosis was calculated as the ratio of the percentage of apoptotic cells among PV-infected IMR5 cells to the percentage of apoptotic cells among mock-infected IMR5 cells. Data are means from two independent experiments. Error bars represent the standard errors of the means. *, P < 0.05 by Student's t test comparing untreated IMR5 cells to treated IMR5 cells.