FIG. 6.

Transcription of PBCV-1-encoded bi-DCD gene and distribution of the bi-DCD genes among chlorella viruses. (A) Total RNAs were isolated from uninfected (H) and PBCV-infected Chlorella NC64A cells at the indicated times (in minutes) and hybridized with a ³²P-labeled A596R gene probe. (B) Hybridization of the PBCV-1 bi-DCD gene to DNAs isolated from the host Chlorella NC64A, 42 viruses that infect Chlorella NC64A, and five viruses (CVA-1, CVB-1, CVG-1, CVM-1, and CVR-1) that infect Chlorella Pbi. The DNAs were hybridized with a ³²P-labeled A596R gene probe. The blots contain 1, 0.5, 0.25, and 0.12 μg DNA from left to right, respectively.