FIG. 5.

Replication potential of TAN1, TAN2, and TAN3 molecular clones in human CD4⁺ T cells. The replication kinetics of TAN1.910, TAN2.1, and TAN3.1 as well as TAN2.69, which encodes a Lys at position 287 of its RT protein, are shown in relation to those of HIV-1 (SG3) and SIVcpzPtt (GAB1.1a) reference strains (y axis, nanograms of RT activity per ml of culture supernatant). Enriched CD4⁺ T cells from four human donors were infected with transfection-derived culture supernatants equilibrated by particle-associated RT activity (50 ng), except for TAN2.1, which was used at maximal RT activity. Results from one representative experiment are shown.