FIG. 5.
Virus replication in IM-MDDCs. IM-MDDCs (1 × 106 cells) were pulsed for 60 min with similar concentrations of isogenic NL4-3balenv either lacking or bearing host-derived LFA-1 (100 ng of p24Gag). After three washes with PBS, the cells were maintained in complete culture medium supplemented with GM-CSF and IL-4. (A) Virus production was assessed by measuring p24Gag levels in the culture supernatants at the indicated time points. D.P.I., day postinfection. (B) The amounts of integrated viral DNA were quantified at day 12 after virus exposure with real-time PCR assays, as described in Materials and Methods. The data shown represent the means ± the SDs of the results for duplicate samples and are representative of at least four independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (C) The percentage of IM-MDDCs positive for both cell surface DC-SIGN and intracellular p24 was quantified by flow cytometry at 9 days postinfection.