FIG. 8.
Biological significance of the LFA-1-mediated effect. NL4-3balenv (10 ng of p24Gag for panel A and 100 ng of p24Gag for panel B) produced in PBMCs was first incubated for 15 min at 4°C with the F(ab′)2 fragments from the listed antibodies (0.5 μg each in panel A and 5 μg each in panel B). The virus-antibody mixture was diluted in four volumes of RPMI medium before being incubated with IM-MDDCs (1 × 105 cells in panel A and 1 × 106 cells in panel B) for 60 min. After three washes with PBS, IM-MDDCs were either divided into three aliquots and cocultured with autologous CD4+ T cells at a DC-to-T-cell ratio of 1:3 (A) or cultured in complete culture medium supplemented with GM-CSF and IL-4 (B). Virus production was evaluated at either day 2 (A) or day 8 (B) by measuring p24Gag levels. The data shown are the means ± the SDs of the results for triplicate samples and are representative of at least two independent experiments. *, P < 0.05; **, P < 0.01.