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. 2007 Jun 21;26(14):3283–3295. doi: 10.1038/sj.emboj.7601768

Figure 9.

Figure 9

Recombinant LTBP-2 markedly increases deposition of DANCE onto fibrillin-1 microfibrils in fibrillin-2-knockdown cells. HSFs were transfected with each RNAi oligo as indicated, cultured in 5% serum containing media for 14 days and fixed. Two days after transfection, recombinant LTBP-2 protein (16 μg/ml) or mock was added to the culture medium. Cells were stained with anti-DANCE (A–F) and anti-fibrillin-1 (G–L) antibodies. (M–R) Superimpositions of (A–F) and (G–L) with DAPI nuclear staining. Scale bars; 60 μm.