Table 1.
Photolyase activities in plant extracts
| Extract | Dimers (106) removed per hr per μg of protein*
|
||
|---|---|---|---|
| Dark† | Light† | Light + UV-B† | |
| WT | 215 (±24) | 783 (±54) | 750 (±66) |
| uvr2-1 | <14 | <10 | <12 |
Three-week-old seedlings were either wrapped in foil for 24 hr (dark), maintained under CW1500 cool-white lamps with mylar filters (light), or supplemented with UV-B from F40UVB lamps (2 kJ·m−2·h−1 unweighted UV-B; 0.6 kJ·UV-BBE·m−2·h−1; light + UV-B) for 24 hr.
*
Means for four trials each. Standard deviations for WT given in parentheses.
†
Using a second set of extracts, mixing WT and uvr2-1 (1:1), yielded 93% (dark), 100% (light), and 102% (+UV-B) of the expected WT values with uvr2-1 samples contributing no activity or inhibition.