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. 2007 Jun 21;26(14):3273–3282. doi: 10.1038/sj.emboj.7601769

Figure 1.

Figure 1

JD fibroblasts can internalize β-VLDL but not LDL. Normal, JD, ARH−/− and LDLR−/− fibroblasts were cultured in lipoprotein poor media for 48 h. (A) Cells were treated with 20 μg/ml Alexa546-labeled LDL or 10 μg/ml DiI-labeled β-VLDL for 2 h at 37°C and visualized by epifluorescent microscopy. (B, C), cells were treated with 10 μg/ml Alexa546-labeled LDL (B) or 5 μg/ml DiI-labeled β-VLDL (C) for 1 h at 4°C, and then shifted to 37°C for the times indicated. Cells were washed, suspended and fixed at 4°C. Mean fluorescent uptake of lipoproteins was determined by flow cytometry and is reported as a percentage of normal cell uptake at 4 h.