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. 2007 Jun 21;26(14):3273–3282. doi: 10.1038/sj.emboj.7601769

Figure 3.

Figure 3

β-VLDL can drive uptake of the LDLR-Y807C of JD fibroblasts. Normal, JD and ARH−/− fibroblasts were cultured in lipoprotein-poor media for 48 h and then treated with monensin alone for 0, 5, 15 or 60 min, or in combination with 20 μg/ml LDL for 5, 15 or 60 min, or in combination with 10 μg/ml βVLDL for 5, 15 or 60 min. Cells were then surface biotinylated and lysed. The biotinylated proteins were isolated from the whole-cell lysates by neutravidin agarose precipitation. (A) Biotinylated proteins were run on 5–17% SDS–PAGE gels, transferred to nylon membranes and immunoblotted for the presence of LDLR or for CD44. (B) The whole-cell lysates were run on 5–17% SDS–PAGE gels, transferred to nylon membranes and immunoblotted for the presence of LDLR.