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JD fibroblasts can target β-VLDL but not LDL to late endosomes. Normal, JD, ARH−/− and LDLR−/− fibroblasts were cultured in lipoprotein-poor media for 48 h and then treated with LDL-gold or β-VLDL-gold for 90 min at 37°C. Arrows indicate multivesicular bodies, a morphologically distinctive late endosome.
