Figure 5.

The HIC⁸¹⁸ sequence is required for β-VLDL uptake by the LDLR-Y807C. LDLR^−/− fibroblasts were transfected with plasmids that direct the expression of GFP (A), GFP-LDLR (B), GFP-LDLR-W792X (C), GFP-LDLR-Y807C (D), GFP-LDLR-Y807C+E812X (E), GFP-LDLR-Y807C+I817X (F), GFP-LDLR-Y807C+G823X (G), GFP-LDLR-Y807C+R829X (H), GFP-LDLR-Y807C+EDE⁸¹⁴>AAA (I), GFP-LDLR-Y807C+EVH⁸¹⁶>AAA (J), GFP-LDLR-Y807C+HIC⁸¹⁸>AAA (K) or GFP-LDLR-Y807C+CHN⁸²⁰>AAA (L). Cells were then plated on coverslips, treated with lipoprotein poor media, incubated at 37°C with DiI-β-VLDL for 2 h and visualized for the presence of β-VLDL (red), GFP- (green) and DAPI-stained nuclei (blue). Shown below the fluorescent images is a diagram detailing the mutations and their effect on lipoprotein uptake.