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. 2007 Jun 28;26(14):3332–3345. doi: 10.1038/sj.emboj.7601776

Figure 2.

Figure 2

L11 interacts with c-Myc in cells. (A, B) Co-IP of ectopic L11 with ectopic c-Myc. H1299 cells were transfected with V5-c-Myc and Flag-L11 individually, or together and subjected to IP with anti-Flag (A) or anti-V5 (B), followed by IB with anti-Flag or anti-V5 antibody. (C) Overexpressed L11 interacts with untagged c-Myc. H1299 cells transfected with untagged c-Myc in the presence or absence of Flag-L11 were immunoprecipitated with anti-Flag antibody, followed by IB with indicated antibodies. (D, E) Endogenous L11 interacts with endogenous c-Myc. H1299 cell lysates were immunoprecipitated with monoclonal anti-c-Myc (C33) (D) or anti-L11 (E) antibody, followed by IB with anti-L11, polyclonal anti-c-Myc (N262) (D), or monoclonal anti-c-Myc (9E10) antibody (E).