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Relative RNA levels for each RGM after siRNA mediated knockdown. Real-time quantitative Taqman RT-PCR analysis was performed to test the effectiveness and specificity of RNA target knockdown in cells transfected with siRNA duplexes. A scramble control was tested in addition to four distinct siRNA duplexes that target RGMa (A), RGMb (B), or RGMc (C). Error bars represent the standard deviation (n = 4).
