Figure 5.

RGM overexpression in C2C12 cells. Cells were transiently transfected with the same total amount of either empty vector, RGMa, RGMc, or RGMa/RGMc, followed by BMP2 treatment, and then assayed for BRE-luc activity. Error bars represent the standard deviation (n = 4). Significantly higher BRE-luc activity was observed in cells co-transfected with RGMa/RGMc than in cells transfected in either RGMa or RGMc alone, as determined by a two-tailed Student's T-test (p value threshold of 0.05). Cells transfected without co-receptors (empty vector) showed significantly lower BRE-luc activity than all other treatments.