Table 3A.

Primers designed to screen the coding sequence of ENaC α, β, and γ genes. Optimum temperatures (°C) for PCR and DHPLC are included in the table.

		Sequence	PCR	DHPLC
ENaC α
Exon 1	Forward	GGTAGCACGGAGCTGGAG	64	63
	Reverse	CCCAGGCTGAGTTCACTCTC
	Forward	AGCTCAATACTGCTTGGTTGG	61	63
	Reverse	GAAGAGCTCCCGGTAGGAG
	Forward	GGGACAAACGTGAAGAGCAG	61	63
	Reverse	CTTGCTTTTGTGCTGCTGAG
Exon 2	Forward	CACTGATCCCCTCCGTGTTA	60	64
	Reverse	TCCATCAGGCCTCTATCTGAA
Exon 3	Forward	GCTCTCTGTCCCTCACCTTG	63	62
	Reverse	ACCACCAAGCATTTCCTGAG
Exon 4	Forward	CACATTGGAGGTGACAGGAA	63	61
	Reverse	CCAAGGTGAGACCCAGAAGA
Exon 5	Forward	GCTGGCCTTGTTCTATCAGG	62	62
	Reverse	CTCCCTTCAGTCTCCTGCTG
Exon 6	Forward	GGTGAAGCCTGAGTCATTCC	63	62
	Reverse	CCAACCTACTTCCCCTCCAT
Exon 7	Forward	GAGGGATGGAGGGGAAGTAG	61	61
	Reverse	AGCCAGCACCTAGGGAAAA
Exon 8	Forward	GGCACCATTGAAATGCTCTT	59	61
	Reverse	ATCAAAGTGCCCAGTTACGG
Exon 9	Forward	GCAGCTGCTTAACCTGGTAGA	61	61
	Reverse	ATGTCCACTTGTGCGTGTGT
Exon 10	Forward	CCATCCCTGTAAACATGAGG	61	61
	Reverse	CCCCAATATCTCCACCAGAA
Exon 11	Forward	TGGTGGAGATATTGGGGAGA	61	61
	Reverse	CAAACCCTTCTGACCCTTCA
Exon 12	Forward	TGACAGGAGGCGCTAGAGT	62	63
	Reverse	AGTAGCATAGGCAGGTGGAG
	Forward	CTCCACTCCAGCTTCCTCCT	62	63
	Reverse	ATCGTTAGCCCCTGTCCTCT
	Forward	TCTCACTTCAGCACATCTTCC	61	63
	Reverse	GGCCTACCCTGGTCTGTCTT
	Forward	ACCCAAAAGCCCCCTTGT	61	61
	Reverse	AGTACACTGTGGGGGTGAGG
	Forward	CCAAAGGCACCATTTCTTTT	59	61
	Reverse	ATGTAGGCGGTGCCTCAG
ENaC β
Exon 2	Forward	CTAGTCTCCAGGCCCATGAC	62	64
	Reverse	CTACTGGAAGGGGCTGGAAT
Exon 3	Forward	CCCCATGTTCCACACTCTTT	60	62
	Reverse	AACAAAAATCGATTGCTACCAG
Exon 4	Forward	TAATACGGTGCTGCCATTCC	61	63
	Reverse	GCATAGATCAGCCTGTGTGC
Exon 5	Forward	CTCCAGCAGAGCAGGACAAT	62	61
	Reverse	GGTCTTTCCGCCCTGTGT
Exon 6	Forward	GGTGATGGCCTCCCTTCTAT	61	61.5
	Reverse	AGGCAGCCTGAACACAAGAG
Exon 7	Forward	GCTCCATGGGGAGGTACATA	63	63
	Reverse	GTCCGGCCCTCATAGGTAAG
Exon 8	Forward	AAAGTCTCTGGGCTCCAAGG	63	61.5
	Reverse	AGAGGCCCCCTTGCCTAAC
Exon 9	Forward	GAGGGAAGACCCCTGGAAG	62	63.5
	Reverse	ATACTGGGTGTCGCTGGAAG
Exon 10	Forward	TCCTGCAAGTGAGTGTGTCC	62	63
	Reverse	AGGGGGAGAAGACCCTCTTT
Exon 11	Forward	AGCATGTGTGTGCGTGTGTA	60	63
	Reverse	CAGCAAGAGCAGTTTGGACA
Exon 12	Forward	CAACTCAGACTGGAGATGAGCA	62	62.5
	Reverse	GGAACCATAACCCCCACCTA
Exon 13	Forward	CCTAGGTGGGGGTTATGGTT	62	64
	Reverse	GCAGCCTCAGGGAGTCATAG
	Forward	CTTCCAGCCTGACACAACC	62	63.5
	Reverse	GCCTGTCTGCTAGGTCAACA
	Forward	CTGAGGGGTTCATAGGGTCA	62	60.5
	Reverse	ATCCTACACCCCAGACATGC
ENaC γ
Exon 2	Forward	AGTCGCAGGCTCCAGAGAT	62	64
	Reverse	GAGGGGCTTTGACATCCAT
Exon 3	Forward	GGAAGGCAACATGAAGAAGC	61	60
	Reverse	ACTGTGAGCCCACCAGCTC
Exon 4	Forward	AAGGACCTACCCTGGCATCT	63	60
	Reverse	CTCAAGGCCTACAGGTGAGC
Exon 5	Forward	ACGCAATGCTTCCTGACTTC	60	60
	Reverse	TGCACTGCTGCTGTCAAGAT
Exon 6	Forward	CCCTGGGGACTGCTTTTT	60	60
	Reverse	TCCATTAGCAGCACCTCCTT
Exon 7	Forward	AGGGGAATCCTCCTATCTGG	61	62
	Reverse	CCTTGGCCTAGATATAGCTTCA
Exon 8	Forward	AGGGAGTTCCCGGTCTCTAC	63	61
	Reverse	GCGTGGCCAAGCTGATTC
Exon 9	Forward	AGATGGTGGAGGTTCCACAG	63	60
	Reverse	GGGAGAAAGGCACAGAGTGA
Exon 10	Forward	ACTGGGGCAGGTAGGACTTT	62	60
	Reverse	GCTTTGGCTGTGTTGCTGTA
Exon 11	Forward	CCAAAGCCAGAGACAGGTTG	59	61
	Reverse	TCGAATGAACGAAAAGGTGA
Exon 12	Forward	ACCTGGCAGGAAGCCAAG	62	61
	Reverse	CCCTCTGGCAGCAAAACTAC
Exon 13	Forward	CCCTGAGTGCAGGATTTATCA	61	64
	Reverse	GTATCTGGGAGGTGGTGTGC
	Forward	GTCAGTGGCACAAAGCCAAG	62	64
	Reverse	AGCTCATAAGTGCCAAGTCCA
	Forward	CCTGCTGTGAACCGGATA	60	60
	Reverse	GGCCAACTGTCTGTCTGAGG
	Forward	GCCAGCTATTGCCTGACAT	60	60
	Reverse	CGCATACTCTCAGTTCAAAGACA
	Forward	GCCAAATGGTATTCCCACAA	59	57
	Reverse	ATTGGACTAGCCTGGGTGCT