Table 1.
Reactions whose directions have to be reversed to restore connectivity of metabolites using mechanism 1.
| (GapFill) identified reversible reactions in E. coli | Corresponding metabolite(s) in E. coli which is (are) fixed | Pathway |
| ADOCBLS | rdmbzi, agdpcbi, adocbi, adocbip | Cofactor and Prosthetic Group Biosynthesis |
| HETZK | 4mhetz | Cofactor and Prosthetic Group Biosynthesis |
| HMPK1 | 4ahmmp | Cofactor and Prosthetic Group Biosynthesis |
| SPODM | o2- | Unassigned |
| DXYLK | dxyl | Cofactor and Prosthetic Group Biosynthesis |
| ACBIPGT | adocbi, adocbip, 5prdmbz | Cofactor and Prosthetic Group Biosynthesis |
| PGLYCP | 2pglyc | Alternate Carbon Metabolism |
| NNDMBRT | 5prdmbz, dmbzid | Cofactor and Prosthetic Group Biosynthesis |
| PEAMNO | peamn | Alternate Carbon Metabolism |
| ALDD19x | peamn | Alternate Carbon Metabolism |
| CRNt7 | crn, gbbtn, ctbt, crncoa*, bbtcoa, ctbtcoa | Transport, Extracellular |
| AP4AH | ap4a | Nucleotide Salvage Pathways |
| 2DGLCNRy | 2dhglcn | Alternate Carbon Metabolism |
| DKGLCNR2x | 2dhglcn, 25dkglcn | Alternate Carbon Metabolism |
| BETALDHx | betald | Unassigned |
| BETALDHy | betald | Unassigned |
| DKGLCNR1 | 25dkglcn, 2dhglcn | Alternate Carbon Metabolism |
| X5PL3E | ap5a, xu5pL, | Alternate Carbon Metabolism |
| DKGLCNR2y | 25dkglcn | Alternate Carbon Metabolism |
| DKGLCNR2x | 25dkglcn, 2dhglcn | Alternate Carbon Metabolism |
| 2DGULRx | 2dhguln | Alternate Carbon Metabolism |
| 2DGULRy | 2dhguln | Alternate Carbon Metabolism |
| GP4GH | gp4g | Nucleotide Salvage Pathways |
| DOGULNR | 23doguln, 3dhguln | Alternate Carbon Metabolism |
| ADOCBIK | adocbi | Cofactor and Prosthetic Group Biosynthesis |
| KG6PDC | 3dgulnp, 3dhguln | Alternate Carbon Metabolism |
| RZ5PP | dmbzid | Cofactor and Prosthetic Group Biosynthesis |
| GPDDA5 | g3pi | Cell Envelope Biosynthesis |
| GPDDA3 | g3ps | Cell Envelope Biosynthesis |
The reactions in bold have support in the EcoCyc database. The pathways in which these reactions are present and the metabolites whose production they enable are as shown. The metabolites shown in bold are fixed by a combination of mechanisms 1 and 2.